Hemoglobin A1c

Written by: Solaris Diagnostics

Glycated or glycosylated hemoglobin is an assay performed in order to diagnose and monitor
treatment for type one and type two diabetes. Most commonly referred to as hemoglobin A1c,
this assay was initially isolated from other types of hemoglobin in 1958 via the use of a
chromatographic column. However, the correlation between diabetes mellitus and hemoglobin
A1c was not discovered until about ten years later.

The hemoglobin-carrying component of blood, the red blood cell, has an average life-span of
three months. In a patient with uncontrolled diabetes, glucose can build up in the blood stream
and bind to hemoglobin, becoming glycated. The higher the glycated hemoglobin, the higher the
patient’s average blood sugar. Glycated hemoglobin is important because it provides medical
health professionals with a three-month average of a patient’s blood glucose level.

Monitoring hemoglobin A1c and controlling daily blood sugar highs and lows can help to
manage diabetes and control symptoms that result from high and low blood sugars. Consistent
elevations in blood sugar can lead to issues such as coronary disease, heart attack, stroke, heart
failure, kidney failure, vision loss, neuropathy, and poor wound healing. The American College
of Endocrinology recommends an A1c of <6.5%, which correlates to an average daily blood
sugar of <=140. A person without diabetes mellitus should have an A1c in the 4%-6% range, or
an average daily sugar between 68-126.

At Solaris Diagnostics, hemoglobin A1c is measured utilizing high-performance liquid
chromatography, a gold standard technology for measuring A1C testing. This test method
requires whole blood samples collected in EDTA tubes and takes approximately 1.6 minutes.
During analysis, hemoglobin in the patient sample will bind to negatively charged resin inside an
analytical cartridge. A buffer gradient is then pumped through the analytical cartridge which aids
in separating out the several different types of hemoglobin in the sample, which all have slightly
different charges. This allows us to isolate the glycated hemoglobin in the sample and determine
the patient’s average blood sugar.

Share this Article

Recent Articles


Testosterone is a hormone that is produced by both males and females which acts as a chemical messenger throughout the body. Testosterone is produced in

Read More »

Hepatitis C

  Hepatitis C is a liver disease caused by the hepatitis C virus (HCV). It is spread through exposure to blood, often as a result

Read More »

TSH Assay

The TSH assay is an in vitro test used for the quantitative determination of TSH in human serum and plasma. The anterior pituitary gland produces

Read More »

Vitamin B12 Assay

The Vitamin B12 assay is an in vitro test used for the quantitative determination of Vitamin B12 in human serum and plasma. Vitamin B12 is

Read More »

Prothrombin Time (PT)

The prothrombin time (PT) is the most commonly used anticoagulant test and was first introduced into use by Dr. Armand Quick and colleagues in 1935.1

Read More »

Otitis Externa

  Otitis externa (also known as “swimmer’s ear”) is inflammation of the external ear canal.  It is often caused by bacterial or fungal infection that

Read More »

Need a Reliable Diagnostic Partner?

At Solaris Diagnostics, we make it easy!

Our Results Portal allows you access patient reports quickly and securely online.

To request access to the Providers Results Portal, please complete our Provider Registration form.

Scroll to Top