Hepatitis B Surface Antigen (HBSAg)

CPT: 87340

Expected Turnaround Time

24 Hours

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Specimen Requirements

Specimen

Serum, Plasma

Volume

5 ml

Minimum Volume

100 ul

Container

SST (Serum), Red Top (Separate Serum), Lithium Heparin (Plasma), EDTA (Plasma), Sodium Heparin (Plasma)

Storage Instructions

Room Temp., Refrigerated (2-8 C)

Stability Requirements

Room temp. 7 days, Refrigerated 7 days

Causes for Rejection

Incorrect labeling, incorrect anticoagulant, frozen gel-barrier tube, mislabeled samples

Test Details

Use

This test is use for the qualitative and quantitative determination of hepatitis B surface antigen in human adult, adolescent, and pediatric serum or plasma.

Methodology

Immunochemiluminometric assay

Reference Interval

Non- Reactive

Additional Information

Hepatitis B Surface Antigen (HBsAg) is a protein on the surface of the hepatitis B virus (HBV) and serves as a key marker of active HBV infection. It is the earliest detectable viral antigen to appear in the blood following exposure to HBV, often present before symptoms arise. A positive HBsAg result indicates either acute or chronic hepatitis B infection, depending on the duration of antigen presence. If HBsAg persists for more than six months, it is classified as a chronic infection. The test is commonly used for diagnostic screening, blood donor testing, prenatal screening, and monitoring of known HBV carriers.  A negative HBsAg, in the absence of other HBV markers, typically indicates no current infection.

References

Terrault, N. A., Lok, A. S. F., McMahon, B. J., Chang, K. M., Hwang, J. P., Jonas, M. M., … & Yawn, B. P. (2018). Update on prevention, diagnosis, and treatment of chronic hepatitis B: AASLD 2018 hepatitis B guidance. Hepatology, 67(4), 1560–1599. https://doi.org/10.1002/hep.29800

Centers for Disease Control and Prevention (CDC). (2024). Hepatitis B questions and answers for health professionals. Retrieved from https://www.cdc.gov/hepatitis/hbv/hbvfaq.htm

Dienstag, J. L. (2008). Hepatitis B virus infection. The New England Journal of Medicine, 359(14), 1486–1500. https://doi.org/10.1056/NEJMra0801644

Footnotes

  1. Kalra A, Yetiskul E, Wehrle CJ, et al. Physiology, Liver. [Updated 2023 May 1]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK535438/   
  2. Sonneveld MJ, Rijckborst V, Cakaloglu Y, et al.. Durable hepatitis B surface antigen decline in hepatitis B e antigen-positive chronic hepatitis B patients treated with pegylated interferon-alpha2b: relation to response and HBV genotype. Antivir Ther 2012; 17:9–17. [PubMed] [Google Scholar] 
  3. Manesis EK, Papatheodoridis GV, Tiniakos DG, et al.. Hepatitis B surface antigen: relation to hepatitis B replication parameters in HBeAg-negative chronic hepatitis B. J Hepatol 2011; 55:61–8. [PubMed] [Google Scholar] 

Statement on Medical Necessity
All ordered tests should be medically necessary for the diagnosis or detection of disease, illness, impairment, symptom, syndrome, or disorder and the results should be used in the medical management and treatment decisions for the patient. Solaris requires ICD-10 codes with each order for lab testing and both the tests ordered and the diagnosis should be documented in the provider’s medical record for the patient. The United States Department of Health and Human Services, Office of Inspector General, takes the position that a provider who orders medically unnecessary tests may be subject to civil penalties.

Panels and Profiles
Solaris offers Providers the convenience of ordering test combinations in a group at times with the flexibility to choose appropriate test(s) for individual patients. Providers should only order those tests that he or she believes are medically necessary for each patient, and a lesser inclusive profile or individual tests should be ordered if not all tests in the test combination/profile are medically necessary. All tests offered in a test combination/profile may be ordered separately as individual tests. Solaris encourages clients to contact their Solaris representative if the testing configurations shown do not meet individual needs for any reason, or if some other combination of procedures is needed.

CPT Codes
CPT Codes listed are in accordance with Current Procedural Terminology, a publication of the American Medical Association. CPT codes are the responsibility of the billing party and are listed here for informational purposes. Correct coding may vary from one carrier to another. Solaris may bill specific carriers using codes other than what is shown.

Questions?

For questions or inquiries related to testing please reach out to
customerservice@solarisdx.com or contact us by phone at (844) 550-0308.